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sodium citrate buffer  (Thermo Fisher)


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    Thermo Fisher sodium citrate buffer
    Sodium Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sodium+citrate+buffer/Sodium+citrate%2C+0%2E5M+buffer+soln%2E%2C+pH+6%2E0/pmc13199671-104-10-42
    Average 95 stars, based on 1 article reviews
    sodium citrate buffer - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Influence of Ploidy and Genetic Background on Stress Tolerance of Intraspecific Yeast Hybrids
    Article Snippet: The pellet was then resuspended in 66 μL of 50 mM sodium citrate buffer containing 0.25 mg/mL RNase A (Thermo Scientific, Vilnius, Lithuania) and 1 mg/mL proteinase K (Thermo Scientific, Vilnius, Lithuania). .. After overnight incubation at 37°C, 33 μL of 50 mM sodium citrate buffer containing 6 μM SYTOX Green (Invitrogen, Eugene, OR, USA) was added. ..

    Article Title: Indoor pollution induced Oxinflammatory responses in lung tissues.
    Article Snippet: .. Antigen retrieval was achieved using heat-based epitope retrieval with 10 mM sodium citrate buffer (AP-9003-500, ThermoFisher Scientific, USA) (pH 6.0) at a sub-boiling temperature in a 500 W microwave for 10 min. After cooling for 20 min, sections were washed 2 times for 5 min in PBS, blocked with 5% BSA in PBS at RT for 45 min, and incubated overnight at 4 °C with primary antibodies for ASC (Cat. Sc-514414, Santa Cruz Biotechnology) 1:200 in 2% BSA in PBS, NLRP3 (Cat. MA5-32255, ThermoFisher Scientific) 1:200 dilution in 2% BSA in PBS, HO-1 (Cat. Sc-390991, Santa Cruz Biotechnology) 1:500 in 2 % BSA and 4-HNE (Cat. AB5605, Sigma Aldrich) 1:400 dilution in 2% BSA in PBS. ..

    Article Title: Multi-omics profiling identifies inosine as a key metabolite associated with embryonic arrest in hydrosalpinx-associated infertility
    Article Snippet: .. For immunofluorescence staining, the sections underwent heat-mediated antigen retrieval with sodium citrate buffer (pH of 6.0) for 16 min, then blocked in 10% normal donkey serum (YEASON) at room temperature for 1 h. Primary antibodies against goat anti-Purine Nucleoside Phosphorylase (PNP) (1:100, Thermo scientific, PA5-37877, RRID: AB_2554485 ), mouse anti-acetylated Tubulin (1:200, Sigma, T7451, RRID: AB_609894 ), rabbit anti-γH2AX (1:200, Cell Signalling Technology, 9718, RRID: AB_2118009 ) and rabbit anti-Cleaved Caspase-3 (Asp175) (1:200, Cell Signalling Technology, 9661, RRID: AB_2341188 ) were diluted in PBS and incubated with sections in a humidified chamber for 16 h at 4 °C. .. After 3 washes in PBS, the sections were incubated with the corresponding secondary antibody conjugated to Alexa Fluor 594 or 488 (Invitrogen; 1:200) and 4’,6-diamidino-2-phenylindole (DAPI) (1:200, ab285390, Abcam) for 1 h at room temperature, followed by three washes with PBS.

    other:

    Article Title: Spatial transcriptomics reveals altered communities and drivers of aberrant epithelia and pro-fibrotic fibroblasts in interstitial lung diseases
    Article Snippet: Excess cells were cryopreserved by resuspension in CryoStor CS10 (Stem Cell Technologies #07930) at 1-2e6 cells/mL.

    Article Title: Comparative Analysis of Streptozotocin, Streptozotocin–Nicotinamide and Alloxan-Based Diabetes Models in Female Wistar Rats
    Article Snippet: Sodium citrate buffer (0.5 M, pH 5.0; 250 mL, Thermo Fisher Scientific, Waltham, MA, USA), 10% glucose solution, distilled water, and sodium chloride solution (NaCl 0.9%) were also used throughout the experimental procedures.

    Reverse Transcription:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    Gene Expression:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    SYBR Green Assay:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    Injection:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    Electron Microscopy:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    Software:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    Electric Cell-substrate Impedance Sensing:

    Article Title: Eicosapentaenoic acid reprograms cerebrovascular metabolism and impairs repair after brain injury, with relevance to chronic traumatic encephalopathy.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER eicosapentaenoic acid (EPA) Cayman Cat # 901110 fatty acid–free BSA Fisher Bioreagents Cat # BP9704-100 NaCl Sigma-Aldrich Cat #S9888 Growth factor–reduced Matrigel Corning Cat # 354230 Calcein-AM Thermo Scientific Cat # 65-0853-39 4-MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide)- M6494 1 g Thermo Scientific Cat #M6494 RNeasy Mini Kit Qiagen Cat # 74104 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems Cat # 4368814 TaqMan Gene Expression Assays Applied Biosystems Cat # 4331182 LightCycler 480 SYBR Green I Master Mix Roche Cat # 04707516001 Forane (Isoflurane), 100mL Isospire N/A Ketamine HCL INJ 100 MG/ML 5 mL VI NDC Cat # 0143-9509-01 Xylazine Injection 100mg/ml, 50 mL Dechra Cat # 07-893-8424 Paraformaldehyde 4% Glutaraldehyde 1% In 0.1M Phosphate Buffer pH7.0 Electron Microscopy Sciences Cat # 15949 Sodium Citrate Buffer (pH = 6) Thermo Scientific Cat #J61815.AK Tri-Sodium Hydrate (MW = 294.1g) Thermo Scientific Cat # AC447292500 Distilled Water Thermo Scientific Cat # 15230204 Deposited Data Raw and analyzed Bulk-RNA Seq data for Mouse This Paper GSE319252 Raw and analyzed Bulk-RNA Seq data for Human This Paper GSE319253 Experimental models: Cell lines Human brain microvascular endothelial cells (HBEC 5i ATCC Cat# CRL 3245 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock No. 000664 Software and algorithms Noldus EthoVision XT software Ethovision N/A Amersham Imager 680 system GE Healthcare N/A QUANTL software GE Healthcare N/A Volocity 6.3 software PerkinElmer N/A Fiji/ImageJ Coloc 2 Fiji/ImageJ N/A Imaris (Bitplane) Oxford Instruments N/A DESeq2 package R (v4.2.2) N/A ClusterProfiler R (v4.2.2) N/A GraphPad Prism GraphPad Software N/A ECIS software Applied BioPhysics (ECIS) N/A 20 Cell Reports ▪▪, 117135, ▪▪, 2026 Article ll OPEN ACCESS ..

    Immunofluorescence:

    Article Title: Multi-omics profiling identifies inosine as a key metabolite associated with embryonic arrest in hydrosalpinx-associated infertility
    Article Snippet: .. For immunofluorescence staining, the sections underwent heat-mediated antigen retrieval with sodium citrate buffer (pH of 6.0) for 16 min, then blocked in 10% normal donkey serum (YEASON) at room temperature for 1 h. Primary antibodies against goat anti-Purine Nucleoside Phosphorylase (PNP) (1:100, Thermo scientific, PA5-37877, RRID: AB_2554485 ), mouse anti-acetylated Tubulin (1:200, Sigma, T7451, RRID: AB_609894 ), rabbit anti-γH2AX (1:200, Cell Signalling Technology, 9718, RRID: AB_2118009 ) and rabbit anti-Cleaved Caspase-3 (Asp175) (1:200, Cell Signalling Technology, 9661, RRID: AB_2341188 ) were diluted in PBS and incubated with sections in a humidified chamber for 16 h at 4 °C. .. After 3 washes in PBS, the sections were incubated with the corresponding secondary antibody conjugated to Alexa Fluor 594 or 488 (Invitrogen; 1:200) and 4’,6-diamidino-2-phenylindole (DAPI) (1:200, ab285390, Abcam) for 1 h at room temperature, followed by three washes with PBS.

    Staining:

    Article Title: Multi-omics profiling identifies inosine as a key metabolite associated with embryonic arrest in hydrosalpinx-associated infertility
    Article Snippet: .. For immunofluorescence staining, the sections underwent heat-mediated antigen retrieval with sodium citrate buffer (pH of 6.0) for 16 min, then blocked in 10% normal donkey serum (YEASON) at room temperature for 1 h. Primary antibodies against goat anti-Purine Nucleoside Phosphorylase (PNP) (1:100, Thermo scientific, PA5-37877, RRID: AB_2554485 ), mouse anti-acetylated Tubulin (1:200, Sigma, T7451, RRID: AB_609894 ), rabbit anti-γH2AX (1:200, Cell Signalling Technology, 9718, RRID: AB_2118009 ) and rabbit anti-Cleaved Caspase-3 (Asp175) (1:200, Cell Signalling Technology, 9661, RRID: AB_2341188 ) were diluted in PBS and incubated with sections in a humidified chamber for 16 h at 4 °C. .. After 3 washes in PBS, the sections were incubated with the corresponding secondary antibody conjugated to Alexa Fluor 594 or 488 (Invitrogen; 1:200) and 4’,6-diamidino-2-phenylindole (DAPI) (1:200, ab285390, Abcam) for 1 h at room temperature, followed by three washes with PBS.



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